Elizabeth Zepeda and Katie Carter

Our BluePippin instrument is used for DNA size selection. Several genomic applications benefit from collecting only DNA fragments within a specified size range from a pool of DNA. This service is often used before Illumina sequencing to remove undesired PCR peaks or before long read sequencing to increase the proportion of fragments greater than a given length in a library.

Using pulsed-field electrophoresis on pre-cast gel cassettes, a DNA sample is separated and fragments within the target range are eluted into buffer. Each cassette can run up to 5 samples at once. Up to 5 ug of DNA can be loaded into each well.

The following cassettes are available at CQLS:

• 3% agarose, 100-250bp
• 2% agarose, 100-600bp
• 1.5% agarose, 250bp – 1.5kb
• 0.75% agarose, 1-50kb

The desired size range must lie within the total size range capability of the cassette. For example, between 200-350 bp on a 2% agarose cassette would be an acceptable range.

The expected collection yield of target sizes is approximately 50-80% based on product validation studies. Yield can vary greatly depending on the desired range and input fragment sizes.

Contact Katie Carter for inquiries about this service.

Source: sagescience.com