Tag Archives: drought stress

Meet the Team: Unmanned Aerial Systems

Sadie Keller

Unmanned Aerial Systems applications in agriculture have interested me from the time I saw some trial flights at the World Ag Expo in Tulare, California in 2020. The possibilities of crop mapping, using multispectral imagery to create NDVI data, and optimizing resources seemed
endless. And while all of this is still true, I have learned over the last couple years that there is a lot more to it than just getting out the drone and flying it over a field.

Dalyn McCauley, captured in a 2022 drone image

In the Fall quarter of 2022, I was able to take a class on campus from Dr. Michael Wing called “Unmanned Aerial Systems Remote Sensing. In this course I was able to get some hands-on experience flying a DJI Phantom 4, a DJI Matrice 200, and a DJI Matrice 300. We also were able to take aerial imagery with a MicaSense Altum multispectral and thermal camera attached to the DJI Matrice 300 and analyze that data during the course of the class. To analyze the data we learned how to use AgiSoft Metashape photogrammetry software to make orthomosaics of our area of interest. With the orthomosaics, we were able to perform different sorts of analysis using ArcGIS Pro software and R. This class really gave me a solid introduction to the collection and analysis of aerial imagery.

DJI matrice 210 with a zen muse xt2 camera flying over red maples
The DJI matrice 210 with a zen muse xt2 camera flying over red maples

With some knowledge under my belt after taking that course, I decided to look into taking the Part 107 Certification exam to obtain my FAA administered Remote Pilot License. The purpose of this certification is to be able to fly a drone for commercial, government, or any other non-
recreational purposes. In the Nackley Lab, we have access to a DJI Matrice 210 quadcopter and a MicaSense Red-Edge M camera so I wanted to be able to open up some more avenues for research by being able to pilot this drone for our lab!

DJI matrice 300 with a micasense altum camera
DJI matrice 300 with a micasense altum camera

In our lab, a lot of our work centers around major challenges to the nursery production industry in Oregon. Working with a drone can allow you to survey a large area for early signs of drought stress, nutrient deficiencies, or pests to minimize a loss in yield. Now that I have the Remote Pilot Certification, my goal is to help our lab create more aerial imagery (data) that ties into our work which addresses major challenges to nursery production in Oregon like irrigation application, pest management, plant nutrition, and climate adaptations.

Thinking about getting a Remote Pilot Certification? Here are Sadie’s tips for Studying (click here).

For more information about the part 107 certification or UAS applications feel free to contact me
@ kellesad@oregonstate.edu.

Other great OSU resources:
BEAV UAS Program – Dr. Kristine Bucklin and Dr. Lloyd Nackley
Aerial Information Systems Laboratory – Dr. Michael Wing

Meet the Team: The Bounty of a Season

Success in Summer 2022

For the past few years we’ve limited gatherings on the farm due to COVID-19 restrictions. In the summer of 2022, however, we were finally able to welcome the public back for Nursery Program Field Days. We’d like to take this opportunity to boast about a few of our highlights from the last several months.

Sadie Keller presenting to growers
Sadie Keller discusses shade tree physiology

For the first time, the Nackley Nursery Production team was an official stop on the Oregon Association of Nurseries Farwest Innovative Production Grower Tour. Our portion of the tour at NWREC showcased sensor-controlled irrigation, heat-stress mitigation techniques, LiDAR smart-sprayer systems, and practices that can reduce boxwood blight spread, and methods of scouting and monitoring insects in nurseries and greenhouses. These projects offer a wide range of savings for growers.: up to 80% improvement in irrigation efficiency, up to 70% reduction in sprayed pesticides, and a significant reduction in boxwood blight infection.

image shows participants examining landscape plants
Stakeholders evaluating climate-readiness of various landscape ornamentals

The second big event was an open house for our Climate Ready Landscape Plant trial, the largest coordinated landscape plant irrigation trial in the Western US. Plant professionals from around the region came to rate plants and discuss how we, as a society, are going to maintain healthy landscapes while faced with increasing extreme weather.

Ongoing projects that will continue this year include, research by our graduate student Sadie Keller, who is investigating Oak and Maple drought tolerance. This summer, Sadie shared her preliminary findings with scientists at the American Society for Horticultural Science, in Chicago.

Sadie Keller and Lloyd Nackley at the ASHS Meeting in Chicago.

In addition, Dr. Melissa Scherr continues our research on the Pacific Flatheaded beetle, with the anticipation of a grower event hosted at NWREC discussing current research on Flathead Borer biology and control this coming April – 2023.

The Nursery Program Team, summer 2022.

Climate-Ready Landscape Plant FIELD DAY

UC Davis Plant Trial Field Day 2020
Photos: UC Davis Plant Trial Field Day 2020 cr: Karrie Reid

Who should attend: Professionals from landscape, horticulture, nursery and related fields; OSU Master Gardeners; garden writers; academics/educators

When: September 14th 2022; 10am – 2pm (Arrive when convenient; ratings take about 60 min)

Where:                  OSU’s North Willamette Research & Extension Center

15210 NE Miley Rd, Aurora, OR 97002

What’s involved: Evaluating aesthetic qualities of selected landscape plants (about 60 minutes).

Photos: UC Davis Plant Trial Field Day 2020 Credit: Karrie Reid
Photos: UC Davis Plant Trial Field Day 2020 Credit: Karrie Reid

About this Event

The ever-changing climate iputs pressure on the industry to develop more sustainable plants. As part of a six-university study, OSU seeks to improve urban water-use efficiency by evaluating landscape plant performance on three irrigation treatments corresponding to the Water Use Classification of Landscape Species (WUCOLS): High, Moderate, and Low categories of water need. The plants are irrigated regularly during their first summer after planting. Treatments are imposed during the second growing season where researchers collect growth and quality ratings.

The Field Day allows landscape, nursery, and horticultural industry professionals and educators the opportunity to see new plants in their 2nd year and share your opinions and preferences by rating a representative sample of the plants in the field undergoing irrigation treatments. One plant from each of the 3 water levels, for 15 different species (some released to the public and some not yet) will be surveyed. Along with this field of 360 plants, you will be able to get a sneak peek at the next year’s field, currently in an establishment phase.

Important Details: The fields are packed dirt/uneven mulch, sturdy comfortable shoes, sunscreen and/or a hat are suggested. At the trial site, you will be provided a ratings sheet, clipboard, pen, and given general instructions when you arrive. It is a self-guided tour among our 720 landscape plants. Lots to look at but only a small sub set to evaluate. Hot Coffee and cold water will be provided. We value your feedback and hope to see you there!

For questions contact:

Brian Hill

(562) 841-1522

brian.hill@oregonstate.edu

Irrigation: Drought Physiology of Ornamental Shade Trees

Sadie Keller

Highlights:

  1. Shade tree growers need to be prepared for the effects of climate change in Oregon.
  2. In order to equip growers with the tools necessary for production success, we aim to determine critical shade tree stress thresholds, characterize plant responses to drought conditions, and correlate remotely collected spectral images with ground based plant water stress measurements.
  3. Previous studies have sought drought response measurements for Acer rubrum (Red Maple) and Quercus rubra (Red Oak), but never in a nursery production setting.
  4. We aim to disseminate this information to Oregon shade tree growers at the completion of this experiment with the hope to aid growers in making data driven irrigation decisions and demonstrate the use of these technologies in nursery production settings.
Sadie in some of the shade trees equipped with soil moisture sensors and a weather station.
Sadie Keller in the shade trees equipped with soil moisture sensors and a weather station.

The Problem:

In Oregon’s Willamette Valley, the heart of the nursery country, rainfall is scarce during the summer and humidity is low. Oregon’s dry summer conditions can lead to low moisture stress conditions for maples and oaks in normal years. Plant stress resulting from low soil moisture, high heat, and low relative humidity have been exacerbated in recent years with the increasing frequency of heatwaves and drought. Drought and heat stress scorch the maple and oak canopies, which can lead to decreased plant quality and economic losses for shade tree growers. Sensor-based technologies can be used to model plant responses to environmental gradients to develop warning systems to help growers prevent stress and bridge a knowledge gap in the nursery production industry regarding drought responses.

How are we studying plant stress responses?

Starting late June 2022, we will implement two irrigation treatments (well-watered and drought) in our shade tree planting with each row having independent irrigation control. The well-watered rows will be maintained at a soil water potential of  >-1.0 mPa. The drought treatment rows will be allowed to naturally dry down to a soil water potential of -4 mPa. If during the experiment, our metrics (stomatal conductance and stem water potential) do not show considerable responses at -4 mPa tension, we will allow the drought treatment to continue to dry down progressively (-1 mPa) until stress is evident.

Why and how do we measure stem water potential?

Plant water status is commonly defined in terms of water potential or the ability of the water to do work. In most cases, well watered plants have “high” water status and drought conditions lead to a “low” water status (Levin and Nackley 2021). Using the pressure chamber, we will take midday stem water potential measurements twice weekly from 12pm-3pm. This time frame is important because it represents the time of day where leaf transpiration is at its maximum.

The pressure chamber
The pressure chamber (https://www.pmsinstrument.com/)

First, we will cover the leaf and stems to be measured with an opaque bag for at least 10 minutes before pressurization to allow the plant to stop transpiring. Once we excise the sample from the tree it should be placed into the pressure chamber or “pressure bomb” within 30 seconds (Levin 2019). Once the stem is placed into the chamber and pressure is applied, the amount of pressure that it takes to cause water to appear at the cut surface tells us how much tension the stem is experiencing.

Why and how do we measure stomatal conductance?

We measure stomatal conductance using a porometer that measures the degree of stomatal openness and the number of stomata (Licor.com). This indicates the plant’s physiological response to its current environment. If a plant is stressed, it will tend to close its stomata and lower the stomatal conductance rate. We will be using a combination of the LI-6800 Portable Photosynthesis System and the LI-600 Porometer/Fluorometer to make our measurements twice a week from 12pm-3pm.

For more information:

Please stay tuned in the coming months for more blog posts about how we will find plant stress thresholds by measuring the hydraulic conductivity of these shade trees. We will also correlate remotely collected spectral and thermal images with our ground based plant stress measurements to demonstrate how implementing a UAS equipped with a multispectral and thermal camera can be used to detect water stress in nursery production.

Irrigation: Going LOCOS for On-Site Weather Data

How we are using low-cost and open-source weather stations for decision support 

Dalyn McCauley

On-farm weather data can provide valuable information to growers including informing irrigation scheduling, tracking plant growth indices, and mitigating damaging events like frost, heat waves or disease. Weather can vary widely across landscapes, even across a single field, and we have found that there is value in having multiple distributed weather stations on-farm to capture variability across small spatial scales. To do this cost effectively, I developed a low-cost open-source weather station (LOCOS) for my M.S. thesis at the University of Idaho that uses low-cost sensors and an Arduino microcontroller for data logging. By distributing multiple LOCOS across a vineyard, we found that there were distinct micro-climates that had varying susceptibility to grape powdery mildew disease. From calculating a Powdery Mildew Risk Index at each station, we saw that some vineyard blocks could benefit from unique fungicide application schedules. You can read more about this project here.

Figure 1: The first iteration of the LOCOS design installed at a vineyard in 2019 (Julieatta, Idaho).
Figure 1: The first iteration of the LOCOS design installed at a vineyard in 2019 (Julieatta, Idaho). 

Since then, the LOCOS have been adapted to study crop water stress. In the summer of 2021, we used LOCOS equipped with infrared thermometers to develop a crop water stress index (CWSI) for hazelnuts. The CWSI is based on leaf temperature and weather data (air temperature, relative humidity, wind speed, and solar radiation). Leaf temperature is a known indicator of plant stress. When a plant is actively transpiring the leaves will be cooler than the surrounding air because of the evaporative cooling effect of transpiration. Whereas a plant that is stressed and not transpiring will have a warmer canopy that is closer to the ambient air temperature. The CWSI varies from 0 to 1, where 1 indicates a stressed, non-transpiring plant, and 0 indicates a well-watered plant transpiring at max potential.  

We used the LOCOS to collect canopy temperature of the hazelnut trees from June to September, 2021. The trees were subject to three different irrigation treatments, over watered, moderate water, and no water (dryland) so we could get a range of canopy temperatures to incorporate into our model. We also collected data on leaf water potential, leaf transpiration and leaf conductance to validate the index against. We found that the CWSI we developed was closely correlated with leaf water potential (r2 = 0.84), leaf conductance (r2 = 0.75) and leaf transpiration (r2 = 0.72). These are exciting results because it shows that the LOCOS could provide continuous data on crop water stress that can be used to inform irrigation decision in near real-time. This summer, we will use the LOCOS in another study to develop a CWSI for red maples. 

Figure 2: LOCOS installed in a hazelnut orchard for CWSI study in 2021 (Aurora, OR).