MB 311 Writing Exercise #7

Adéle Mennerat and Ben C. Sheldon in their microbial ecological study titled “How to Deal with PCR contamination in molecular Microbial Ecology” (2014) state that the use of enzymes such as DNase1 and Sau3AI decontaminate DNA in PCR reagents. Mennerat and Sheldon provide evidence of this via the use of generalized models with Poisson distribution, and ran ANOSIM on a similarity matrix showing if the use of these enzymes was affective or not when applied to infected DNA. The purpose of this study is to show whether or not the use of enzymes is effective in order to provide a possible solution to PCR contamination. The authors establish a solid scholarly integrity with the microbiological scientific community that uses PCR analysis on a daily basis.

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