No one loves mushrooms as much as Ray Van Court loves mushrooms.

Their favorite food? Matsutake mushrooms. Their favorite hobby? Mushroom hunting. Their favorite time of the year? Mushroom season.

In fact, Van Court loves mushrooms so much they quit their corporate job to pursue ways to make the world a better place through fungi.

As a PhD candidate in wood science and graduate research assistant, Van Court is working on a project with assistant professor of forest-based bio-products Gerald Presley. Together, they use ectomycorrhizal fungi to bioremediate heavy metal-treated wood waste.

“Preservatives are critical to retaining the structural integrity of wood, but disposal of treated wood is problematic,” Van Court says. “Wood treated with metals including arsenic and copper is disposed of in landfills, often unlined, where these toxic metals can move into the environment. Preventing the migration of these metals, and potentially recovering them, could reduce the ecological impact of these contaminants.”

Certain species of ectomycorrhizal fungi are known to tolerate high metal environments, and initial work has shown that they may reduce metal toxicity. These mechanisms include binding them, transporting them, and producing compounds that stabilize the metals. Introducing fungi particularly adept at immobilizing metals in contaminated sites could reduce the environmental impact of toxic metal migration. The resulting retention of bound metals may also allow for reclamation.

This, says Van Court, represents a long-term solution to the problem of treated wood waste with little required inputs – all ectomycorrhizal fungi need is trees to associate with.

To test this idea, Van Court and Presley are performing a multi-stage lab experiment, screening 20 different species of ectomycorrhizal fungi in plate culture against three toxic metals.

“This screening will identify which species best tolerate and uptake metals used in wood preservatives and is an enormous increase in species and metals compared to previous research,” says Van Court.

In the second stage of the research, trees will be inoculated with the best performing fungi and planted in heavy metal-treated mesocosms, controlled containers that replicate natural environments. Trees and fungi will grow together in the metal contaminated system for a few months, after which their effect on metal will be measured. This initial work will test the effectiveness of the fungal system and pave the way for future field research.

While doing the research, Van Court was surprised by the scarcity of technologies related to ectomycorrhizal fungi and the limited knowledge on fungi growth. The fungi are usually in symbiosis with trees and for many species very little is known regarding how to replicate what the tree or other organisms in the ecosystem typically provide to the fungus.

“Admittedly, they are much harder to grow and maintain than decay fungi, but they represent a lot of untapped potential,” Van Court says. “As all kinds of products – from medicines to packaging material – have come from decay fungi, what new sustainable products might come from ectomycorrhizal ones? With new analytical and genetic tools, I think we are poised to learn much more about these fungi, and I am excited to see where this research and other projects can go.”

A version of this story appeared in the Spring 2022 issue of Focus on Forestry, the alumni magazine of the Oregon State University College of Forestry.

Nathan Vega, an undergraduate student double majoring in renewable materials and forestry, has always had an interest in the fields of renewable energy and forest-based bioenergy.

“I am especially interested in biochar for its potential to help with wildfire prevention, energy production and agricultural management,” Vega says.

Biochar is a carbon-rich, charcoal-like substance made by burning organic material, like agricultural or forestry waste, at low oxygen levels in a process called pyrolysis. Biochar can be used as a soil enhancer or as a way to sequester carbon. The energy or heat created during the conversion can also be captured and used as clean energy.

“Biochar is part of something called the circular economy,” Vega says. “And the foundation of this economy is a transition to renewable energy and materials.”

An alternative to the traditional linear economy, the circular economy is restorative or regenerative by design. It seeks to reduce waste and material use, recover resources at the end of a product’s life, and channel them back into production, significantly reducing pressure on the environment.

Vega jumped at an opportunity to work within the circular economy, assisting Scott Leavengood, director of Oregon Wood Innovation Center, in testing Portland, Oregon, based Sankofa Lumber’s new line of panel products known as “SecondStory.”

“SecondStory” panels are unique in that they are composed of reclaimed structural building materials, including lumber, oriented strand board (OSB) and plywood. Sankofa refers to these panels as architectural surfaces and advises using them for purposes like flooring, casework and wall cladding. “SecondStory” panels are currently installed in the Oregon State women’s gymnastics facility locker room.

Leavengood and Vega tested the panels to determine qualities like hardness, bond and bending strength and moisture performance. They measured the panels’ performance based on comparable products like
particleboard, medium-density fiberboard (MDF) and hardwood plywood. The Cascadia CleanTech Accelerator, powered by VertueLab and CleanTech Alliance, funded the testing.

“For entrepreneurs working with any kind of new material or new product, the first question they always get from potential customers is ‘what’s it like?’ or in other words, how does the product compare to what’s on the market now?’ says Leavengood. “We were able to help Sankofa Lumber answer these questions since Nathan put the product through a workout.”

Bond strength is critical for composite products. Leavengood and Vega found the strength excellent even after products were exposed to high humidity and water submersion for several days.

Focusing on his classes and assisting Leavengood with his research projects provided Vega with support and something to focus on during the pandemic.

“Everyone at the College of Forestry was very welcoming and friendly,” Vega says, “Plus, this job was a great part of the last year-and-a-half as it let me get out of the house and listen to music while I did the experiments.”

Vega is a recipient of the Friends of Renewable Materials Seneca Scholarship, Powers Scholarship, and Presidential Scholarship from the College of Forestry. He said receiving the scholarships has been essential to ensuring his success at Oregon State.

“These scholarships have allowed me to pursue my education without distraction or worry,” Vega says. “It’s been such a relief to find that I am so supported.”

When Vega is not studying, he likes to spend his free time reading, gardening, cooking, listening to music, hiking and playing the drums. He also likes to spend his time with his friends and family and he recently joined the college’s logging sports team.

After graduation, Vega wants to work in bioenergy, specifically biochar production from forest biomass as a carbon-negative energy source.

A version of this story appeared in the Spring 2022 issue of Focus on Forestry, the alumni magazine of the Oregon State University College of Forestry.