Adèle Mennerat and Ben C. Seheldon, in their paper entitles “How to Deal with PCR Contamination in Molecular Microbial Ecology,” assert that enzymatic treatment of PCR reagents help reduce contamination. The authors provide evidence that two enzymes, DNase 1 and SauAI both reduce contamination but DNase also shows substantial reduction in bacterial richness while SauAI does not affect richness and simultaneously reduces contamination. The purpose of this paper is to encourage use of decontaminating reagents in order to start setting this as standard procedure. The authors establishes a scholarly relationship with academic in microbial ecology as well as other biological sciences.
Mennerat, A., Sheldon, B.C. How to Deal with PCR Contamination in Molecular Microbial Ecology. Microb Ecol 68, 834–841 (2014).
