(1) Microbiologist, Adele Mennerat, in her research article entitled “How to Deal with PCR Contamination in Molecular Microbial Ecology” (2014) asserts that enzyme Sau3AI when used to decontaminate PCR reagents can cause higher accuracy in PCR reads when compared to using enzyme DNase 1. (2) Mennerat provides evidence that Sau3AI is the more effective enzyme by comparing similarity in community composition, and finding that Sau3AI was more similar to the original structure. (3) The purpose of this research is to demonstrate that an enzyme is needed to clean up PCR reagents in order for the results to have less decomtanition. (4) Mennerat establishes an instructive and scholarly relationship with microbiologist and any professional that wants to use PCR.
Mennerat A, Sheldon BC. How to deal with PCR contamination in molecular microbial ecology. Microb Ecol. 2014 Nov;68(4):834-41. doi: 10.1007/s00248-014-0453-y. Epub 2014 Jul 9. PMID: 25004997.
