1) Adele Mennerat and Ben C. Sheldon in their research article entitled “How to Deal with PCR Contamination in Molecular Microbial Ecology” (2014) asserts that the presence of contaminant bacterial DNA may have consequences in the interpretation of ecological data, especially when low levels of template DNA from highly diverse communities is used, thus advocating for the use of decontaminating procedures to become standard procedure. 2) Mennerat and Sheldon have provided evidence that enzymatic treatment by DNase 1, DTT, and Sau3AI reduce contamination levels. 3) The purpose of this research article is to solve issues with contamination muddling results in the molecular microbial ecology field. 4) Mennerat and Sheldon establish a scholarly relationship with audiences in the molecular microbial ecology community (as well as the general scientific community, since all scientific disciplines that use PCR can have issues that stem from contamination).
