Adele Mennerat in their research paper titled “How to Deal with PCR Contamination in Molecular Microbial Ecology”(2014) outlines strategies for reducing bacterial contamination in PCR samples. Mennerat provides evidence that using restrictions enzymes Dnase 1 and Sau3 AI to PCR samples can reduce bacterial richness observed, thereby reducing overall contamination, and works the most effectively to combat contamination when compared to other strategies such as UV radiation of samples. The purpose of their research is to explore the effectiveness of differing strategies to reduce bacterial contamination, in order to improve the quality of PCR samples and resulting data collected for future experiments. Mennerat establishes a connection to both microbiologist and ecologist researchers alike, presenting their work in a academic fashion that involves several of the biological sciences.
