{"id":2998,"date":"2019-09-24T00:10:18","date_gmt":"2019-09-24T00:10:18","guid":{"rendered":"http:\/\/blogs.oregonstate.edu\/gemmlab\/?p=2998"},"modified":"2019-09-24T00:10:24","modified_gmt":"2019-09-24T00:10:24","slug":"the-significance-of-blubber-hormone-sampling-in-conservation-and-monitoring-of-marine-mammals","status":"publish","type":"post","link":"https:\/\/blogs.oregonstate.edu\/gemmlab\/2019\/09\/24\/the-significance-of-blubber-hormone-sampling-in-conservation-and-monitoring-of-marine-mammals\/","title":{"rendered":"The significance of blubber hormone sampling in conservation and monitoring of marine mammals"},"content":{"rendered":"\n<p><strong>By:&nbsp;<\/strong><a href=\"https:\/\/mmi.oregonstate.edu\/people\/alexa-kownacki\"><strong>Alexa Kownacki<\/strong><\/a><strong>, Ph.D. Student, OSU Department of Fisheries and Wildlife, Geospatial Ecology of Marine Megafauna Lab<\/strong><\/p>\n\n\n\n<p>Marine mammals are challenging to study for many reasons, and\nspecifically because they inhabit the areas of the Earth that are uninhabited\nby people: the oceans. Monitoring marine mammal populations to gather baselines\non their health condition and reproductive status is not as simple as trap and\nrelease, which is a method often conducted for terrestrial animals. Marine\nmammals are constantly moving in vast areas below the surface. Moreover,\ncetaceans, which do not spend time on land, are arguably the most challenging\nto sample. <\/p>\n\n\n\n<p>One component of <a href=\"https:\/\/mmi.oregonstate.edu\/gemm-lab\/comparative-health-assessment-bottlenose-dolphin-ecotypes-california\">my project<\/a>, based in California, USA, is a health assessment analyzing hormones of the bottlenose dolphins that frequent both the coastal and the offshore waters. Therefore, I am all too familiar with the hurdles of collecting health data from living marine mammals, especially cetaceans. However, the past few decades have seen major advancements in technology both in the laboratory and with equipment, including one tool that continues to be critical in understanding cetacean health: blubber biopsies. <\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/Biopsy-dart-below-dorsal-Ttru.jpeg\" alt=\"\" class=\"wp-image-3000\" width=\"400\" height=\"286\" \/><figcaption>Biopsy dart hitting a bottlenose dolphin below the dorsal fin. Image Source: NMFS<\/figcaption><\/figure><\/div>\n\n\n\n<p>Blubber biopsies are typically obtained via low-powered crossbow with a bumper affixed to the arrow to de-power it once it hits the skin. The arrow tip has a small, pronged metal attachment to collect an eraser-tipped size amount of tissue with surface blubber and skin. I compare this to a <a href=\"https:\/\/www.mayoclinic.org\/tests-procedures\/skin-biopsy\/about\/pac-20384634\">skin punch biopsies<\/a> in humans; it\u2019s small, minimally-invasive, and requires no follow-up care. With a small team of scientists, we use small, rigid-inflatable vessels to survey the known locations of where the bottlenose dolphins tend to gather. Then, we assess the conditions of the seas and of the animals, first making sure we are collecting from animals without potentially lowered immune systems (no large, visible wounds) or calves (less than one years old). Once we have photographed the individual\u2019s dorsal fin to identify the individual, one person assembles the biopsy dart and crossbow apparatus following sterile procedures when attaching the biopsy tips to avoid infection. Another person prepares to photograph the animal to match the biopsy information to the individual dolphin. One scientist aims the crossbow for the body of the dolphin, directly below the dorsal fin, while the another photographs the biopsy dart hitting the animal and watches where it bounces off. Then, the boat maneuvers to the floating biopsy dart to recover the dart and the sample. Finally, the tip with blubber and skin tissue is collected, again using sterile procedures, and the sample is archived for further processing. A similar process, using an air gun instead of a crossbow can be viewed below:<\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube aligncenter wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<span class=\"embed-youtube\" style=\"text-align:center; display: block;\"><iframe loading=\"lazy\" class=\"youtube-player\" width=\"660\" height=\"372\" src=\"https:\/\/www.youtube.com\/embed\/FvcxnhOAA14?version=3&#038;rel=1&#038;showsearch=0&#038;showinfo=1&#038;iv_load_policy=1&#038;fs=1&#038;hl=en-US&#038;autohide=2&#038;wmode=transparent\" allowfullscreen=\"true\" style=\"border:0;\" sandbox=\"allow-scripts allow-same-origin allow-popups allow-presentation allow-popups-to-escape-sandbox\"><\/iframe><\/span>\n<\/div><figcaption>GEMM Lab members using an air gun loaded with a biopsy dart to procure marine mammal blubber from a blue whale in New Zealand. Video Source: GEMM Laboratory.<\/figcaption><\/figure>\n\n\n\n<p>Part of the biopsy process is holding ourselves to the highest standards in our minimally-invasive technique, which requires constant practice, even on land. <\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/IMG_1058-768x1024.jpg\" alt=\"\" class=\"wp-image-3002\" width=\"580\" height=\"773\" srcset=\"https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/IMG_1058-768x1024.jpg 768w, https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/IMG_1058-225x300.jpg 225w, https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/IMG_1058.jpg 1537w\" sizes=\"auto, (max-width: 580px) 100vw, 580px\" \/><figcaption>Alexa practicing proper crossbow technique on land under supervision. Image Source: Alexa Kownacki <\/figcaption><\/figure><\/div>\n\n\n\n<p>Blubber is the lipid-rich, vascularized tissue under the\nepidermis that is used in thermoregulation and fat storage for marine mammals. Blubber\nis an ideal matrix for storing lipophilic (fat-loving) steroid hormones because\nof its high fat content. Steroid hormones, such as cortisol, progesterone, and\ntestosterone, are naturally circulating in the blood stream and are released in\nhigh concentrations during specific events. Unlike blood, blubber is less\ndynamic and therefore tells a much longer history of the animal\u2019s nutritional\nstate, environmental exposure, stress level, and life history status. Blubber\nis the cribs-notes version of a marine mammal\u2019s biography over its previous few\nmonths of life. Blood, on the other hand, is the news story from the last 24\nhours. Both matrices serve a specific purpose in telling the story, but blubber\nis much more feasible to obtain from a cetacean and provides a longer time\nframe in terms of information on the past. <\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/blubber.png\" alt=\"\" class=\"wp-image-3004\" width=\"365\" height=\"344\" \/><figcaption>A simplified depiction of marine mammal blubber starting from the top (most exterior surface) being the skin surface down to the muscle (most interior). Image Source: schoolnet.org.za<\/figcaption><\/figure><\/div>\n\n\n\n<p>I use blubber biopsies for assessing cortisol, testosterone,\nand progesterone in the bottlenose dolphins. Cortisol is a glucocorticoid that\nis frequently associated with stress, including in humans. Marine mammals\nutilize the same hypothalamic-pituitary-adrenal (HPA) axis that is responsible\nfor the fight-or-flight response, as well as other metabolic regulations.\nDuring prolonged stressful events, cortisol levels will remain elevated, which\nhas long-term repercussions for an animal\u2019s health, such as lowered immune\nsystems and decreased ability to respond to predators. Testosterone and\nprogesterone are sex hormones, which can be used to indicate sex of the\nindividual and determine reproductive status. This reproductive information\nallows us to assess the population\u2019s composition and structure of males and\nfemales, as well as potential growth or decline in population (West et al.\n2014). <\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/Alexa_Biopsy-768x1024.jpg\" alt=\"\" class=\"wp-image-3003\" width=\"580\" height=\"773\" srcset=\"https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/Alexa_Biopsy-768x1024.jpg 768w, https:\/\/osu-wams-blogs-uploads.s3.amazonaws.com\/blogs.dir\/2115\/files\/2019\/09\/Alexa_Biopsy-225x300.jpg 225w\" sizes=\"auto, (max-width: 580px) 100vw, 580px\" \/><figcaption> Alexa using a crossbow from a small boat off of San Diego, CA. Image Source: Alexa Kownacki<\/figcaption><\/figure><\/div>\n\n\n\n<p>The coastal and offshore bottlenose dolphin ecotypes of interest in my research occupy different locations and are therefore exposed to different health threats. This is a primary reason for conducting health assessments, specifically analyzing blubber hormone levels. The offshore ecotype is found many kilometers offshore and is most often encountered around the southern Channel Islands. In contrast, the coastal ecotype is found within 2 kilometers of shore (Lowther-Thieleking et al. 2015) where they are subjected to more human exposure, both directly and indirectly, because of their close proximity to the mainland of the United States. Coastal dolphins have a higher likelihood of fishery-related mortality, the negative effects of urbanization including coastal runoff and habitat degradation, and recreational activities (Hwang et al. 2014). The blubber hormone data from my project will inform which demographics are most at-risk. From this information, I can provide data supporting why specific resources should be allocated differently and therefore help vulnerable populations. Further proving that the small amount of tissue from a blubber biopsy can help secure a better future for population by adjusting and informing conservation strategies. <\/p>\n\n\n\n<p><strong>Literature Cited:<\/strong><\/p>\n\n\n\n<p>Hwang, Alice, Richard H Defran, Maddalena Bearzi, Daniela. Maldini, Charles A Saylan, Aime \u0301e R Lang, Kimberly J Dudzik, Oscar R Guzo n-Zatarain, Dennis L Kelly, and David W Weller. 2014. \u201cCoastal Range and Movements of Common Bottlenose Dolphins (Tursiops Truncatus) off California and Baja California, Mexico.\u201d <em>Bulletin of the Southern California Academy of Sciences<\/em> 113 (1): 1\u201313. https:\/\/doi.org\/10.3390\/toxins6010211.<\/p>\n\n\n\n<p>Lowther-Thieleking, Janet L.,\nFrederick I. Archer, Aimee R. Lang, and David W. Weller. 2015. \u201cGenetic\nDifferentiation among Coastal and Offshore Common Bottlenose Dolphins, Tursiops\nTruncatus, in the Eastern North Pacific Ocean.\u201d <em>Marine Mammal Science<\/em> 31\n(1): 1\u201320. https:\/\/doi.org\/10.1111\/mms.12135.<\/p>\n\n\n\n<p>West, Kristi L., Jan Ramer, Janine L. Brown, Jay Sweeney, Erin M. Hanahoe, Tom Reidarson, Jeffry Proudfoot, and Don R. Bergfelt. 2014. \u201cThyroid Hormone Concentrations in Relation to Age, Sex, Pregnancy, and Perinatal Loss in Bottlenose Dolphins (Tursiops Truncatus).\u201d <em>General and Comparative Endocrinology<\/em> 197: 73\u201381. https:\/\/doi.org\/10.1016\/j.ygcen.2013.11.021.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>By:&nbsp;Alexa Kownacki, Ph.D. Student, OSU Department of Fisheries and Wildlife, Geospatial Ecology of Marine Megafauna Lab Marine mammals are challenging to study for many reasons, and specifically because they inhabit the areas of the Earth that are uninhabited by people: the oceans. Monitoring marine mammal populations to gather baselines on their health condition and reproductive &hellip; <a href=\"https:\/\/blogs.oregonstate.edu\/gemmlab\/2019\/09\/24\/the-significance-of-blubber-hormone-sampling-in-conservation-and-monitoring-of-marine-mammals\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">The significance of blubber hormone sampling in conservation and monitoring of marine mammals<\/span><\/a><\/p>\n","protected":false},"author":8612,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1011750],"tags":[1310489,712828,1310490,1237884,1310488,1237832,712796,1237645,97092,635061,140586,635445,1237681,513,626,1237778,336,155,97272,638029,5],"class_list":["post-2998","post","type-post","status-publish","format-standard","hentry","category-bottlenose-dolphin-population-health","tag-baselines","tag-biopsy","tag-biopsy-dart","tag-blubber","tag-blubber-hormones","tag-blue-whales","tag-bottlenose-dolphin","tag-bottlenose-dolphins","tag-california","tag-cetaceans","tag-conservation","tag-gemm-lab","tag-hormones","tag-marine-mammals","tag-monitoring","tag-nmfs","tag-noaa","tag-oregon-state-university","tag-phd","tag-san-diego","tag-science"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"post_mailing_queue_ids":[],"_links":{"self":[{"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/posts\/2998","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/users\/8612"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/comments?post=2998"}],"version-history":[{"count":6,"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/posts\/2998\/revisions"}],"predecessor-version":[{"id":3008,"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/posts\/2998\/revisions\/3008"}],"wp:attachment":[{"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/media?parent=2998"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/categories?post=2998"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.oregonstate.edu\/gemmlab\/wp-json\/wp\/v2\/tags?post=2998"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}