Outline Lecture #21 Spring 2017

Biotechnology Techniques

Purification and Detection

  • Separation – HERE
  • Detection by UV light of stained DNA or radioactive labeling

Restriction Endonucleases

  • Cutting – HERE
  • Restriction/Modification – HERE
  • Cleavage Sites – HERE

“Cloning”

  • Making Recombinant DNA – HERE
  • Virus Cloning
  • Cell Cloning – HERE
  • Plasmids
    • pET Expression Vectors – HERE
    • pBR 322 HERE
    • pUC19 – HERE
  • Multiple Cloning Site – HERE
  • Inserting DNA Fragments into a Virus HERE
  • Selecting for Recombinant DNAs with a Plasmid – HERE
  • Blue-White Screening – HERE

Genetic Engineering

  • Mutant Mosquitoes
  • Transgenic mustard, tomatoes, crops
  • Insulin Synthesis in engineered bacteria/yeast – HERE
  • Histidine Tagging – HERE
  • Making a DNA Library – HERE
  • Making cDNA – HERE
  • PCR – HERE
  • Southern Blotting
  • DNA Fingerprinting – HERE
  • RFLP Analysis
  • Paternity Testing – HERE

DNA Sequencing

  • Dideoxynucleotides – HERE
  • Sequence Determination – HERE and HERE
  • RNA Interference – HERE and HERE

Genomics / Proteomics

  • Microarrays – HERE
  • Expression Profiles tell relative gene expression in a treated cell compared to a control

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