Monthly Archives: April 2018

Antibiotic resistance: The truth lies in the sludge

 

Genevieve experiencing Vietnamese culture at Sam Mountain in the Mekong Delta

Did you know that about 30% of people here in Oregon have septic tanks? Why is that relevant to this week’s topic you ask? Our guest this week on Inspiration Dissemination, Genevieve Schutzius is an Environmental engineering masters student in the College of Engineering interested in waste water management. Genevieve is working with Dr. Tala Navab-Daneshmand as part of the Navab lab. The lab’s mission is to identify the fate and transmission pathways of pathogenic and antibiotic-resistant bacteria from wastewater systems to environmental reservoirs, and to design engineered systems and interventions to reduce the associated human health risks.

 

 

A beautiful sunrise over the Saigon River in District 4 of Ho Chi Minh City.

Recently, Genevieve spent a term abroad working on a project that is in collaboration with Dr. Mi Nguyen at Nguyen Tat Thanh University in Vietnam. The purpose of the study is to identify the human health risks associated with the spread of infectious bacteria resistant to antibiotics in areas with high septic tank use. Specifically, Genevieve’s project is to identify the fate of antibiotic resistance in soils and waters as recipients of untreated septic sludge.

 

Genevieve sampling a sludge-filled canal using a fashioned “sampling stick” from an abandoned bamboo fishing pole in the northwest of Ho Chi Minh City.

She did this by collecting 55 soil samples from canals, rivers, parks, and fields in Ho Chi Minh City, then plated dilutions of these samples to quantify the number of E. coli, which is a common indicator of fecal contamination. She selected E. coli colonies and brought them back to her lab at OSU, where she performed the disk diffusion method. The disk diffusion method involves plating isolated bacteria across an entire agar plate and see how it grows in the presence of disks containing antibiotics. She tested them against 9 different antibiotics, finding that 69% of 129 isolates were resistant to more than two! She is also conducting a microcosm study to see how resistant bacteria thrives in soils and in different temperature environments. Soon, she will determine the presence of absence of antibiotic-resistant genes in her isolated bacteria using PCR to amplify genes.

Samples mixed with bacteria including chosen E. coli isolates (circled).

Why Vietnam? Well Vietnam has high levels of septic tank use and out of 11 Asian countries surveyed, Vietnam also had the highest levels of antibiotic resistance in patients due to the ease at which they are acquired. A survey Genevieve assisted in implementing while in Vietnam opened her eyes to just how easy it is to get antibiotics and how much they are used among citizens.

 

A plate showing how resistant this particular E.coli isolate is to ampicillin (full resistance), streptomycin (full resistance), gentamicin (mostly resistant), and imipenem (not resistant – “last resort” antibiotic.

 

Originally from Colorado, Genevieve acquired her undergraduate degree in environmental engineering at the University of Colorado Boulder where she became interested in waste water management. She always knew that she wanted to end up in the pacific northwest and after finding out about Oregon State Universities program she decided that the environmental engineering program suited her interests. Following completion of her masters degree she hopes to continue to travel and find work in the humanitarian/non-profit public health and sanitation sector.

In Genevieve’s free time, she enjoys experimenting with her cooking, typically with different types of Indian spices. She also enjoys partaking in activities such as yoga, snowboarding, playing piano, and singing.

 

Tune in to 88.7 FM at 7:00 PM Sunday evening to hear more about Genevieve and her research on antibiotic resistance in areas of high septic tank use, or stream the program live.

Beyond doom and gloom: highlighting solutions to ocean acidification

When we hear news coverage of global environmental changes, it can easily overwhelm us. We mentally curl up into the fetal position and conclude there is nothing we can do to stave off the changes that Earth is projected to experience. One of these changes is ocean acidification–a phenomenon where carbon dioxide in the atmosphere is absorbed by the ocean. As carbon dioxide levels increase in our atmosphere, more of it is able to dissolve into the ocean and lower its pH, making it more acidic. A decrease of 0.1 pH unit in the global ocean since the beginning of the 1900s may not seem like a lot, but because pH is represented on a logarithmic scale, it actually represents about a 30% increase in hydrogen ions. This makes it harder for organisms like oysters, clams, and corals to build hard shells and skeletons. It is uncertain how this phenomenon could affect the long-term fate of these organisms, as well as the fish that depend on them.

Brian flying in a hot air balloon north of Mt. Rainer, WA.

This is where Brian Erickson comes in. Brian, a masters student in Marine Resource Management in OSU’s College of Earth, Ocean, and Atmospheric Science, observed that most curricula designed to teach high school students about ocean acidification do not discuss actionable solutions that most people can take in their everyday lives to mitigate their carbon footprints. Do student attitudes change when presented with solutions like insulating homes to save on heat, swapping incandescent bulbs with LEDs, or consolidating trips to the store to minimize gas consumption?

Brian at work during his first field biology job, studying the sexual reproduction of tropical seaweeds in St. Croix, U.S. Virgin Islands and San Blas, Panama. It’s easy to fall in love with the ocean when you snorkel on coral reefs for two summers!

A former high school science teacher himself, Brian grew up in St. Louis and received his undergraduate degree in biology from Lewis and Clark College. As an undergraduate, he first became acquainted with environmental research as a field technician in St. Croix in the Caribbean. After participating in Teach For America in New York City, he took many environmental research and education jobs before deciding to return to the ocean to bridge his interests of outdoor education and social science. As his masters draws to a close, Brian will be staying at OSU to begin a PhD in Fisheries and Wildlife working to bring multiple perspectives to marine conservation efforts in East Africa.

Helping students dissect a shark at Bronx Career & College Preparatory High School (Bronx, NY).

Taking students on their first canoe trip with Parks in Focus near Pictured Rocks, MI.

To hear more about Brian’s research and experiences in education, tune in to KBVR Corvallis 88.7 FM at 7 pm on April 15th, or stream it online here. If you’re busy at that time, the show will appear on our podcast later this week.

 

Genes & Body Metabolism: How our Muscles Control Outcomes

The basic human body plan is fairly similar (most have eyes, arms, and legs) but how efficiently our bodies’ function is unique and depend heavily on our genes. Although our brains use a lot of the simple energy compounds (like glucose), our skeletal muscles use 70% of our body’s total energy production such as fats, sugars, and amino acids. All of this energy demand from our skeletal muscles means our body’s metabolism is highly regulated by our muscles. If you want a higher metabolism then you should work out more to gain muscle; this process of muscle formation or repair is a complicated sequence of events requiring hundreds of genes all working together at the right time to promote muscle development. However, if one or many genes do not function properly this sequence of events have inefficiencies that diminish our muscle production capability; for some this means more time at the gym but for others it could lead to diseases like diabetes.

Vera working with her mouse models to better understand how a body’s metabolism is controlled by their genes

Our guest this evening is Vera Lattier (Chih-Ning Chang) who is a PhD candidate in the Molecular and Cellular Biology Program focusing on one gene in particular that orchestrates the muscle formation process at various stages of life development. This PITX2 gene is implicated in regulating the activity of other genes as well as formation of the eyes, heart, limbs, and abdominal muscles during embryonic stages. During later stages of life the amount of skeletal muscle you have dictates your bodies metabolism, and if you are unable to build muscles you tend to have a lower metabolism that encourages excess food to be stored as fat. This is the first step towards obesity and is also a precursor to developing diabetes that affects nearly 26 million people in the United States. Although eating right and exercising can have a substantial impact to your health, if your genes are not functioning correctly poor health may ensue at no fault of the patients.

Vera’s research uses mice as models to better understand this complex interaction between our genes and our body’s metabolism. As part of a decade’s long research through Dr. Chrissa Kioussi’s research lab at Oregon State University they examined the role of this PITX2 gene in three main stages of muscle formation. By mutating the gene to affect it’s expression (effectively ‘turning off’ the gene) during early embryonic formation the mice bodies were unable to effectively create the physical structures for basic bodily functions and they were not viable embryos. When mutating the gene near the time of birth the mice were fully functional at the early stage of life and seemed normal. However, when they grew older they quickly became obese, in fact three times as heavy as the average mice, that lead to fatty liver disease, enlargement of the heart, obesity, and of course diabetes. Vera’s work continues to try and elucidate the mechanisms behind the connection of our genes and our body’s metabolism through structural muscle formation that could help us to identify these limitations earlier and help save lives.

Vera giving presentations to scientific conferences to help people understand the importance of muscle in body metabolism.

There is so much more to discuss with Vera on tonight’s show. You’ll hear about her first experience with a microscope at a young age and how she dreamed of one day becoming an evil scientist (luckily her parents changed her mind). Be sure to tune in for what is sure to be an enlightening discussion on Sunday April 8th at 7PM on KBVR Corvallis 88.7FM or by listening live.